Pengaruh Kondisi Ekstraksi Terhadap Aktivitas Antibakteri Caulerpa lentilifera terhadap Human Pathogenic Bacteria secara In-vitro

Dea Nathania Hendryanti, Lindayani .

Abstract


Latar belakang: Caulerpa lentilifera atau dikenal juga sebagai anggur laut merupakan jenis rumput laut yang tersebar luas di Indonesia (nama lokal: Lato) namun, studi aktivitas antibakteri bahan pangan tersebut masih kurang diteliti.

Tujuan penelitian: Penelitian ini bertujuan untuk mengetahui pengaruh waktu ekstraksi (1, 2, 3 hari) dan rasio sampel terhadap pelarut (1: 5, 1:10, 1:15) terhadap aktivitas antibakteri ekstrak C. lentilifera kering. Selain itu, penelitian ini juga bertujuan untuk mengetahui total kandungan fenolik dan korelasinya terhadap aktivitas antibakteri

Metode: Penelitian ini menggunakan metode agar-disk diffusion untuk mengetahui aktivitas antibakteri secara in-vitro yang kemudian dilanjutkan dengan uji minimum inhibitory concentration (MIC) dan uji minimum bactericidal concentration (MBC). Metode Folin-Ciocalteu digunakan untuk mengetahui total kandungan fenolik.

Hasil: Uji antibakteri menunjukkan aktivitas tertinggi, baik melawan bakteri Gram negatif maupun Gram positif (Bacillus cereus, Escherichia coli, Salmonella thypimurium dan Staphylococcus aureus) ketika sampel dimaserasi selama 72 jam dengan rasio sampel terhadap pelarut 1:15. Nilai MIC berkisar 1,5 - 6 mg/mL dengan rasio MBC : MIC kurang dari 4 yang merepresentasikan kemampuan C. lentilifera sebagai inhibitor kuat dan agen bakterisidal. Hasil pengamatan juga menunjukkan adanya korelasi yang sangat kuat (koefisien korelasi = 0,594) dan signifikan (Sig. = 0,001) antara kandungan total fenolik dengan aktivitas antibakteri.

Kesimpulan: Penelitian ini memberikan bukti bahwa Caulerpa lentilifera memiliki potensi yang menjanjikan sebagai agen antibakteri alami melawan bakteri patogen penyebab penyakit pada manusia dimana, salah satu komponen bioaktifnya adalah senyawa fenolik


Keywords


aktivitas antibakteri, Caulerpa lentilifera, total komponen fenolik

References


Vallinayagam K, Arumugam R, Ragupathi R, Kannan R, Thirumaran G, Anantharaman P, editors. Antibacterial Activity of Some Selected Seaweeds from Pudumadam Coastal Regions. 2009.

Hellio C, Bremer G, Pons A, Gal Y, Bourgougnon N. Inhibition of the development of microorganisms (bacteria and fungi) by extracts of marine algae from Brittany, France. Applied microbiology and biotechnology. 2000;54:543-9.

Mary A, Matias J. Rediscovery of naturally occurring seagrape Caulerpa lentillifera from the Gulf of Mannar and its mariculture. Current Sci. 2009;97:1418-20.

Alencar D, Silva S, Pires-Cavalcante K, Lima R, Pereira F, Sousa M, et al. Antioxidant potential and cytotoxic activity of two red seaweed species, Amansia multifida and Meristiella echinocarpa, from the coast of Northeastern Brazil. Anais da Academia Brasileira de Ciencias. 2014;86:251-63.

Choudhury S, Sree A, Mukherjee S, Pattnaik P, Maringanti B. In Vitro Antibacterial Activity of Extracts of Selected Marine Algae and Mangroves against Fish Pathogens. Asian Fisheries Science 18 (2005): 285-294 Asian Fisheries Society, Manila, Philippines. 2005;18.

Cacace JE, Mazza G. Mass transfer process during extraction of phenolic compounds from milled berries. Journal of Food Engineering. 2003;59(4):379-89.

Naczk M, Shahidi F. Extraction and analysis of phenolics in food. Journal of Chromatography A. 2004;1054(1):95-111.

Meireles MA. Extracting Bioactive Compounds for Food Products: Theory and Applications2008.

Pushparaj A, R S R, Thangasamy B. An antibacterial activity of the green seaweed Caulerpha sertularioides using five different solvents. International Journal of PharmTech Research. 2014;6:1-5.

Wikler MA, Cockerill FR, Craig WA, Dudley MN, Eliopoulos GM, Hecht DW, et al. Performance standards for antimicrobial disk susceptibility tests; approved standard - ninth edition. CLSI. 2006;26:1-35.

Yasurin P, Pitinidhipat N. Antibacterial Activity of Chrysanthemum indicum, Centella asiatica and Andrographis paniculata against Bacillus cereus and Listeria monocytogenes under Osmotic Stress. AU Journal of Technology. 2012;15:239-45.

Valgas C, Souza SMD, Smânia E, Smânia A. Screening methods to determine antibacterial activity of natural products. Brazilian Journal of Microbiology. 2007;38:369-80.

Smallwood IM. Handbook of Organic Solvent Properties2012. 1-306 p.

Manilal A, Sugathan S, Selvin J, Shakir C, Kiran S. Antibacterial activity of Falkenbergia hillebrandii (Born) from the Indian coast against human pathogens. Phyton. 2009;78:161-6.

Yi W, Wetzstein H. Anti-tumorigenic activity of five culinary and medicinal herbs grown under greenhouse conditions and their combination effects. Journal of the science of food and agriculture. 2011;91:1849-54.

Rajaei A, Barzegar M, Hamidi Esfahani Z, Sahari MA. Optimization of Extraction Conditions of Phenolic Compounds from Pistachio (Pistachia vera) Green Hull through Response Surface Method. J Agr Sci Tech. 2010;12.

Xu Y, Zhang R, Fu H. Studies on the Optimal Process to Extract Flavonoids from Red-raspberry Fruits. Nature and Science. 2005;3.

Sampath M. Optimization of the extraction process of phenolic antioxidant from Polyalthia longifolia (Sonn.) Thawaites. Journal of applied pharmaceutical science. 2013;3(2):148.

Lavanya R, Veerappan N. Antibacterial Potential of Six Seaweeds Collected from Gulf of Mannar of Southeast Coast of India. Advances in Biological Research. 2011;5.

Salem W, Galal H, El-Deen F. Screening for antibacterial activities in some marine algae from the red sea (Hurghada, Egypt). African journal of microbiology research. 2011;5:2160-7.

Silhavy TJ, Kahne D, Walker S. The bacterial cell envelope. Cold Spring Harb Perspect Biol. 2010;2(5):14.

Hui YH, Kitts D, Stanfield PS. Foodborne disease handbook, second edition, revised and expanded: Volume 4: Seafood and environmental toxins2018. 1-660 p.

Barros L, Calhelha RC, Vaz JA, Ferreira ICFR, Baptista P, Estevinho LM. Antimicrobial activity and bioactive compounds of Portuguese wild edible mushrooms methanolic extracts. European Food Research and Technology. 2007;225(2):151-6.

Thirunavukkarasu R, Pandiyan P, Balaraman D, Subramanian K, George EGJ, Manikkam S, et al. Isolation of bioactive compound from marine seaweeds against fish pathogenic bacteria Vibrio alginolyticus (VA09) and characterisation by FTIR. Journal of Coastal Life Medicine. 2013;1.

Pankey G, Sabath LD. Clinical Relevance of Bacteriostatic versus Bactericidal Mechanisms of Action in the Treatment of Gram‐Positive Bacterial Infections. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 2004;38:864-70.




DOI: https://doi.org/10.24167/vit.v1i1.2967

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