QUANTIFICATION OF BIOACTIVE COMPOUNDS IN FREEZE-DRIED FERMENTED BITTER MELON (Momordica charantia L.) EXTRACT USING Lactobacillus fermentum LLB3
Abstract
Bitter melon (Momordica charantia L.) is one of the plant-based functional foods, which has many health benefits potential due to its bioactive compounds. The usage of Lactobacillus fermentum LLB3 to ferment bitter melon juice has been studied to increase its functional activities. However, the amount of bioactive compounds contained in the fermented juice have not been studied. Therefore, this research provides the quantification of bioactive compounds, including total phenolic and total flavonoid contained in fermented bitter melon juice. Bitter melon fruit was juiced, filtered, and pasteurized at 80°C for 5 minutes. Fermentation was done by using Lactobacillus fermentum LLB3 with incubation times of 24, 48, and 72 hours. The samples were freeze-dried, and the bioactive components were quantified using the spectrophotometry (UV-Vis) method. All data obtained were analyzed statistically to determine the significance of the difference. Both total phenolic and total flavonoid contents were found to be increased by the pasteurization proses due to the thermal treatment applied. However, the fermentation process did not perform any increase either in total phenolics or total flavonoids. Furthermore, the freeze-drying process did not give a significant effect on flavonoid contents, but higher values of phenolic content were obtained.
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Adebiyi, J. A., Obadina, A. O., Adebo, O. A., & Kayitesi, E. (2017). Comparison of nutritional quality and sensory acceptability of biscuits obtained from native, fermented, and malted pearl millet (Pennisetum glaucum) flour. Food Chemistry, 232, 210–217. https://doi.org/10.1016/j.foodchem.2017.04.020
Adetuyi, F. O., & Ibrahim, T. A. (2014). Effect of Fermentation Time on the Phenolic, Flavonoid and Vitamin C Contents and Antioxidant Activities of Okra (Abelmoschus esculentus) Seeds. Nigerian Food Journal, 32(2), 128–137. https://doi.org/10.1016/s0189-7241(15)30128-4
Alavi Rafiee, S., Farhoosh, R., & Sharif, A. (2018). Antioxidant Activity of Gallic Acid as Affected by an Extra Carboxyl Group than Pyrogallol in Various Oxidative Environments. European Journal of Lipid Science and Technology. https://doi.org/10.1002/ejlt.201800319
Blainski, A., Lopes, G. C., & De Mello, J. C. P. (2013). Application and analysis of the folin ciocalteu method for the determination of the total phenolic content from limonium brasiliense L. Molecules, 18, 6852–6865. https://doi.org/10.3390/molecules18066852
Bryman, A., & Cramen, D. (2005). Quantitative Data Analysis with SPSS 12 and 13: A Guide for Social Scientists. Retrieved from https://books.google.co.id/books?hl=en&lr=&id=GHMgxwYHbREC&oi=fnd&pg=PR13&dq=spss+13+analysis&ots=ldstkOZzZH&sig=zQzYV208Df-gPMN-LA2VbT6mt6k&redir_esc=y#v=onepage&q=spss 13 analysis&f=false
Butler, M. J., & Barrientos, R. M. (2020). The impact of nutrition on COVID-19 susceptibility and long-term consequences. Brain, Behavior, and Immunity, 87(April), 53–54. https://doi.org/10.1016/j.bbi.2020.04.040
Chidi, B. S., Bauer, F. F., & Rossouw, D. (2018). Organic acid metabolism and the impact of fermentation practices on wine acidity - A review. South African Journal of Enology and Viticulture, 39(2). https://doi.org/10.21548/39-2-3172
Choi, M. Y., Chai, C., Park, J. H., Lim, J., Lee, J., & Kwon, S. W. (2011). Effects of storage period and heat treatment on phenolic compound composition in dried Citrus peels (Chenpi) and discrimination of Chenpi with different storage periods through targeted metabolomic study using HPLC-DAD analysis. Journal of Pharmaceutical and Biomedical Analysis, 54(4), 638–645. https://doi.org/10.1016/j.jpba.2010.09.036
Chumroenphat, T., Somboonwatthanakul, I., Saensouk, S., & Siriamornpun, S. (2021). Changes in curcuminoids and chemical components of turmeric (Curcuma longa L.) under freeze-drying and low-temperature drying methods. Food Chemistry, 339(September 2020), 128121. https://doi.org/10.1016/j.foodchem.2020.128121
de las Rivas, B., Rodríguez, H., Anguita, J., & Muñoz, R. (2019). Bacterial tannases: classification and biochemical properties. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-018-9519-y
Degrain, A., Manhivi, V., Remize, F., Garcia, C., & Sivakumar, D. (2020). Effect of lactic acid fermentation on color, phenolic compounds and antioxidant activity in african nightshade. Microorganisms, 8. https://doi.org/10.3390/microorganisms8091324
Deshaware, S., Gupta, S., Singhal, R., & Variyar, P. S. (2019). Influence of different pasteurization techniques on antidiabetic, antioxidant and sensory quality of debittered bitter gourd juice during storage. Food Chemistry, 285(November 2018), 156–162. https://doi.org/10.1016/j.foodchem.2019.01.140
Filannino, P., Bai, Y., Di Cagno, R., Gobbetti, M., & Gänzle, M. G. (2015). Metabolism of phenolic compounds by Lactobacillus spp. during fermentation of cherry juice and broccoli puree. Food Microbiology, 46, 272–279. https://doi.org/10.1016/j.fm.2014.08.018
Ghasemzadeh, A., Jaafar, H. Z. E., & Rahmat, A. (2010). Antioxidant activities, total phenolics and flavonoids content in two varieties of malaysia young ginger (Zingiber officinale Roscoe). Molecules, 15(6), 4324–4333. https://doi.org/10.3390/molecules15064324
Ghelichkhani, G., Modaresi, M. H., Rashidi, L., Shariatifar, N., Homapour, M., & Arabameri, M. (2019). Effect of the spray and freeze dryers on the bioactive compounds of olive leaf aqueous extract by chemometrics of HCA and PCA. Journal of Food Measurement and Characterization, 13(4), 2751–2763. https://doi.org/10.1007/s11694-019-00196-3
González, C. M., Llorca, E., Quiles, A., Hernando, I., & Moraga, G. (2020). Water sorption and glass transition in freeze-dried persimmon slices. Effect on physical properties and bioactive compounds. Lwt, 130(May), 109633. https://doi.org/10.1016/j.lwt.2020.109633
Hartajanie, L., Fatimah-Muis, S., Heri-Nugroho Hs, K., Riwanto, I., & Sulchan, M. (2020). Probiotics Fermented Bitter Melon Juice as Promising Complementary Agent for Diabetes Type 2: Study on Animal Model. Journal of Nutrition and Metabolism, 2020. https://doi.org/10.1155/2020/6369873
Hartajanie, L., Lindayani, L., Novita, A., Sutanto, T. E., & Sundoro, A. A. (2018). Lactobacillus fermentum LLB3 improves antioxidant activity of bitter melon (Momordica charantia). Microbiology Indonesia, 12(2), 65–68. https://doi.org/10.5454/mi.12.2.5
Heredia-Castro, P. Y., Reyes-Díaz, R., Rendón-Rosales, M. Á., Beltrán-Barrientos, L. M., Torres-Llanez, M. J., Estrada-Montoya, M. C., … Vallejo-Cordoba, B. (2021). Novel bacteriocins produced by Lactobacillus fermentum strains with bacteriostatic effects in milk against selected indicator microorganisms. Journal of Dairy Science, 104(4), 4033–4043. https://doi.org/10.3168/jds.2020-19531
Hewavitharana, G. G., Perera, D. N., Navaratne, S. B., & Wickramasinghe, I. (2020). Extraction methods of fat from food samples and preparation of fatty acid methyl esters for gas chromatography: A review. Arabian Journal of Chemistry, 13(8), 6865–6875. https://doi.org/10.1016/j.arabjc.2020.06.039
Hsieh, H. J., Lin, J. A., Chen, K. T., Cheng, K. C., & Hsieh, C. W. (2021). Thermal treatment enhances the α-glucosidase inhibitory activity of bitter melon (Momordica charantia) by increasing the free form of phenolic compounds and the contents of Maillard reaction products. Journal of Food Science, 86(7), 3109–3121. https://doi.org/10.1111/1750-3841.15798
Huynh, N. T., Van Camp, J., Smagghe, G., & Raes, K. (2014). Improved release and metabolism of flavonoids by steered fermentation processes: A review. International Journal of Molecular Sciences, pp. 19369–19388. https://doi.org/10.3390/ijms151119369
Islam, S., Jalaluddin, M., & Hettiarachchy, N. S. (2011). Bio-active compounds of bitter melon genotypes (Momordica charantia L.) in relation to their physiological functions. Functional Foods in Health and Disease, 1(2), 61–74. https://doi.org/10.31989/ffhd.v1i2.139
James, S., Nwabueze, T. U., Ndife, J., Onwuka, G. I., & Ata’Anda Usman, M. (2020). Influence of fermentation and germination on some bioactive components of selected lesser legumes indigenous to Nigeria. Journal of Agriculture and Food Research. https://doi.org/10.1016/j.jafr.2020.100086
Karam, M. C., Petit, J., Zimmer, D., Baudelaire Djantou, E., & Scher, J. (2016). Effects of drying and grinding in production of fruit and vegetable powders: A review. Journal of Food Engineering, 188, 32–49. https://doi.org/10.1016/j.jfoodeng.2016.05.001
Kidoń, M., & Grabowska, J. (2021). Bioactive compounds, antioxidant activity, and sensory qualities of red-fleshed apples dried by different methods. Lwt, 136, 110302. https://doi.org/10.1016/j.lwt.2020.110302
Kim, T. J., Silva, J. L., & Jung, Y. S. (2011). Enhanced functional properties of tannic acid after thermal hydrolysis. Food Chemistry, 126(1), 116–120. https://doi.org/10.1016/j.foodchem.2010.10.086
Lange, K. W. (2021). Food science and COVID-19. Food Science and Human Wellness, 10(1), 1–5. https://doi.org/10.1016/j.fshw.2020.08.005
Liu, L., Zhang, C., Zhang, H., Qu, G., Li, C., & Liu, L. (2021). Biotransformation of polyphenols in apple pomace fermented by β-glucosidase-producing lactobacillus rhamnosus l08. Foods, 10(6), 1343. https://doi.org/10.3390/foods10061343
Lotito, S. B., Zhang, W. J., Yang, C. S., Crozier, A., & Frei, B. (2011). Metabolic conversion of dietary flavonoids alters their anti-inflammatory and antioxidant properties. Free Radical Biology and Medicine, 51. https://doi.org/10.1016/j.freeradbiomed.2011.04.032
Lou, S. N., Lin, Y. S., Hsu, Y. S., Chiu, E. M., & Ho, C. T. (2014). Soluble and insoluble phenolic compounds and antioxidant activity of immature calamondin affected by solvents and heat treatment. Food Chemistry, 246–253. https://doi.org/10.1016/j.foodchem.2014.04.009
Maghsoudlou, Y., Asghari Ghajari, M., & Tavasoli, S. (2019). Effects of heat treatment on the phenolic compounds and antioxidant capacity of quince fruit and its tisane’s sensory properties. Journal of Food Science and Technology, 56(5), 2365–2372. https://doi.org/10.1007/s13197-019-03644-6
Mahindrakar, K. V., & Rathod, V. K. (2020). Ultrasonic assisted aqueous extraction of catechin and gallic acid from Syzygium cumini seed kernel and evaluation of total phenolic, flavonoid contents and antioxidant activity. Chemical Engineering and Processing - Process Intensification, 149(December 2019), 107841. https://doi.org/10.1016/j.cep.2020.107841
Mazlan, F. A., Suffian, M., & Sharifuddin, Y. (2015). Biotransformation of Momordica charantia fresh juice by Lactobacillus plantarum BET003 and its putative anti-diabetic potential. PeerJ, 2015(10), 1–18. https://doi.org/10.7717/peerj.1376
Murota, K., Nakamura, Y., & Uehara, M. (2018). Flavonoid metabolism: The interaction of metabolites and gut microbiota. Bioscience, Biotechnology and Biochemistry, pp. 600–610. https://doi.org/10.1080/09168451.2018.1444467
Murphy, S. L., Kochanek, K. D., Xu, J., & Arias, E. (2021). Mortality in the United States, 2020. NCHS Data Brief, (427), 1–8. https://doi.org/10.15620/CDC:112079
Myagmardorj, B., Purev, M. E., & Batdorj, B. (2018). Functional properties of fermented soymilk by lactobacillus fermentum BM-325. Mongolian Journal of Chemistry, 19(45), 32–37. https://doi.org/10.5564/mjc.v19i45.1087
DOI: https://doi.org/10.24167/jfcn.v1i1.11552
DOI (PDF): https://doi.org/10.24167/jfcn.v1i1.11552.g2468
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